| John Troy Donovan | ... | CBI Ron (15 episodes) |
| Emily Swallow | ... | Kim Fischer (14 episodes) |
| Pruitt Taylor Vince | ... | J.J. LaRoche (13 episodes) |
| Josie Loren | ... | Michelle Vega (12 episodes) |
| Lisbeth Kingsley | ... | FBI Special Agent Childs (10 episodes) |
| Rebecca Wisocky | ... | Brenda Shettrick (9 episodes) |
| Michael Rady | ... | Luther Wainwright (9 episodes) |
| Eric Winter | ... | Agent Craig O'Loughlin (8 episodes) |
| David Norona | ... | Oscar Ardiles (8 episodes) |
| Samaire Armstrong | ... | Summer Edgecombe (8 episodes) |
| Kevin Corrigan | ... | Robert Kirkland (7 episodes) |
| Pedro Pascal | ... | Agent Marcus Pike (7 episodes) |
| Terry Kinney | ... | Sam Bosco (6 episodes) |
| Jillian Bach | ... | Sarah Harridan (6 episodes) |
| Catherine Dent | ... | FBI Agent Susan Darcy (6 episodes) |
| Xander Berkeley | ... | Sheriff McAllister (5 episodes) |
| Malcolm McDowell | ... | Bret Stiles (5 episodes) |
| Karl Sonnenberg | ... | CBI Undercover Agent (5 episodes) |
| Reed Diamond | ... | CBI Agent Ray Haffner (5 episodes) |
| Drew Powell | ... | FBI Agent Reede Smith (5 episodes) |
| Emmanuelle Chriqui | ... | Lorelei Martins (5 episodes) |
| Leslie Hope | ... | Kristina Frye (4 episodes) |
| Sean McClay | ... | CSI Man (4 episodes) |
| Dominic Hoffman | ... | Agent Hicks (4 episodes) |
| JoNell Kennedy | ... | Coroner Pat (4 episodes) |
| William Mapother | ... | Richard Haibach (4 episodes) |
| Jim Gunter | ... | Agent Jackson (4 episodes) |
| Robert Picardo | ... | Jason Cooper (4 episodes) |
| Shannon McClung | ... | Agent Collins (4 episodes) |
| Jack Plotnick | ... | Brett Partridge (3 episodes) |
| Angela Martinez | ... | TV Reporter (3 episodes) |
| Maxine Bahns | ... | Anglea Ruskin Jane (3 episodes) |
| Alicia Witt | ... | Rosalind Harker (3 episodes) |
| George Wyner | ... | Coroner Steiner (3 episodes) |
| Shauna Bloom | ... | Rebecca (3 episodes) |
| David Warshofsky | ... | Donny Culpepper (3 episodes) |
| Kassandra Carrington | ... | Mimi (3 episodes) |
| Morena Baccarin | ... | Erica Flynn (3 episodes) |
| Joshua Bitton | ... | CBI Agent Rick Tork (3 episodes) |
| Henry Ian Cusick | ... | Tommy Volker (3 episodes) |
